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    • Home
    • About
    • Genetic Engineering
    • Antibody Discovery
    • PARTNERSHIP
    • Careers
    • Contact
  • Home
  • About
  • Genetic Engineering
  • Antibody Discovery
  • PARTNERSHIP
  • Careers
  • Contact

Genetic Engineering

Leveraging our knowledge and expertise in molecular biology, synthetic biology, genetics, genomics and protein science as well as innovative genetic engineering technologies, we have developed a highly efficient pipeline to generate a broad variety of mutations in mice, ranging from simple knockouts to megabase targeted gene replacements, providing valuable research tools and disease models for mechanistic studies and preclinical testing for therapeutic interventions. 

Selected Publications

  1. Ramalingam N, Jin SX, Moors TE, Fonseca-Ornelas L, Shimanaka K, Lei S, Cam HP, Watson AH, Brontesi L, Ding L, Hacibaloglu DY, Jiang H, Choi SJ, Kanter E, Liu L, Bartels T, Nuber S, Sulzer D, Mosharov EV, Chen WV, Li S, Selkoe DJ, Dettmer U. (2023) Dynamic physiological α-synuclein S129 phosphorylation is driven by neuronal activity. NPJ Parkinsons Dis 9(1):4.  
  2.  Singh S, Serwer L, DuPage A, Elkins K, Chauhan N, Ravn M, Buchanan F, Wang L, Krimm M, Wong K, Sagert J, Tipton K, Moore SJ, Huang Y, Jang A, Ureno E, Miller A, Patrick S, Duvur S, Liu S, Vasiljeva O, Li Y, Henriques T, Badagnani I, Jeffries S, Schleyer S, Leanna R, Krebber C, Viswanathan S, Desnoyers L, Terrett J, Belvin M, Morgan-Lappe S, Kavanaugh WM, Richardson J. (2022) Nonclinical Efficacy and Safety of CX-2029, an Anti-CD71 Probody-Drug Conjugate. Mol Cancer Ther 21(8):1326-1336. 
  3. Zhou L, Wang Y, Wan Q, Wu F, Barbon J, Dunstan R, Gauld S, Konrad M, Leys L, McCarthy R, Namovic M, Nelson C, Overmeyer G, Perron D, Su Z, Wang L, Westmoreland S, Zhang J, Zhu R, Veldman G. (2021) Non-clinical Evaluation on Risankizumab and other IL-23 Antibodies. mAb, 13(1):1964420 
  4. Pao, P.-C., Patnaik, D., Watson, L.A., Gao, F., Pan, L., Wang, J., Adaikkan, C., Penney, J., Cam, H.P., Huang, W.-C., et al. (2020) HDAC1 modulates OGG1-initiated oxidative DNA damage repair in the aging brain and Alzheimer’s disease. Nat Communication 11, 2484. 
  5. Blanchard, J.W., Bula, M., Davila-Velderrain, J., Akay, L.A., Zhu, L., Frank, A., Victor, M.B., Bonner, J.M., Mathys, H., Lin, Y.-T., Ko., T., Bennett, D.A., Cam, H.P., Kellis, M., Tsai, LH. (2020) Reconstruction of the human blood-brain barrier in vitro reveals a pathogenic mechanism of APOE4 in pericytes. Nat Med 26, 952–963. 
  6. Su Z, Paulsboe S, Wetter J, Salte K, Kannan A, Mathew S, Horowitz A, Gerstein C, Namovic M, Todorović V, Seagal J, Edelmayer RM, Viner M, Rinaldi L, Zhou L, Leys L, Huang S, Wang L, Sadhukhan R, Honore P, McGaraughty S, Scott VE. (2019) IL‐36 receptor antagonistic antibodies inhibit inflammatory responses in preclinical models of psoriasiform dermatitis. Exp Derm 28: 113-120  
  7. Lin, Y.-T., Seo, J., Gao, F., Feldman, H.M., Wen, H.-L., Penney, J., Cam, H.P., Gjoneska, E., Raja, W.K., Cheng, J., et al. (2018) APOE4 Causes Widespread Molecular and Cellular Alterations Associated with Alzheimer’s Disease Phenotypes in Human iPSC-Derived Brain Cell Types. Neuron 98, 1141-1154 
  8. Li XL, Li GH, Fu J, Fu YW, Zhang L, Chen W, Arakaki C, Zhang JP, Wen W, Zhao M, Chen WV, Botimer GD, Baylink D, Aranda L, Choi H, Bechar R, Talbot P, Sun CK, Cheng T, Zhang XB (2018) Highly efficient genome editing via CRISPR-Cas9 in human pluripotent stem cells is achieved by transient BCL-XL overexpression. Nucleic Acids Res 46:10195-10215   
  9. Stockmann H, Todorovic V, Richardson PL, Marin V, Scott V, Gerstein C, Lake M, Wang L, Sadhukhan R, and Vasudevan A. (2017) Cell-Surface Receptor–Ligand Interaction Analysis with Homogeneous Time-Resolved FRET and Metabolic Glycan Engineering: Application to Transmembrane and GPI-Anchored Receptors. JACS 139:16822-16829 
  10. Chen WV, Nwakeze CL, Denny CA, O’Keeffe S, Rieger MA, Mountoufaris G, Kirner A, Dougherty JD, Hen R, Wu Q, Maniatis T (2017) Pcdhac2 is required for axonal tiling and assembly of serotonergic circuitries in mice. Science 356: 406-411   
  11. Mountoufaris G*, Chen WV*, Hirabayashi Y, O’Keeffe S, Chevee M, Nwakeze CL, Polleux F, Maniatis T (2017) Multi-cluster Pcdh diversity is required for mouse olfactory neural circuit assembly. Science 356: 411-414 *co-first author   
  12. Murton, H.E., Grady, P.J.R., Chan, T.H., Cam, H.P. *, and Whitehall, S.K*. (2016) Restriction of Retrotransposon Mobilization in Schizosaccharomyces pombe by Transcriptional Silencing and Higher-Order Chromatin Organization. Genetics 203, 1669–1678 
  13. Ye J, Beetz N, O'Keeffe S, Tapia JC, Macpherson L, Chen WV, Bassel-Duby R, Olson EN, Maniatis T (2015) hnRNP U protein is required for normal pre-mRNA splicing and postnatal heart development and function. Proc Natl Acad Sci U S A 112: E3020-29   
  14. Thu CA, Chen WV, Rubinstein R, Chevee M, Wolcott HN, Felsovalyi KO, Tapia JC, Shapiro L, Honig B, Maniatis T (2014) Single-cell identity generated by combinatorial homophilic interactions between a, b, and g protocadherins. Cell 158: 1045-1059   
  15. David R. Lorenz, Lauren F. Meyer, Patrick J.R. Grady, Michelle M. Meyer, Hugh P. Cam. (2014) Heterochromatin assembly and transcriptome repression by Set1 in coordination with a class II histone deacetylase. 10.7554/eLife.04506, ELife 3: e04506   
  16. Mikheyeva, I.V., Grady, P.J.R., Tamburini, F.B., Lorenz, D.R., Cam. H.P. (2014) Multifaceted genome control by Set1 dependent and independent of H3K4 methylation and the Set1C/COMPASS complex. PLOS Genetics 10(10): e1004740 
  17. Chen WV, Alvarez FJ, Lefebvre JL, Friedman B, Nwakeze C, Geiman E, Smith C, Thu CA, Tapia JC, Tasic B, Sanes JR, Maniatis T (2012) Functional significance of isoform diversification in the protocadherin gamma gene cluster. Neuron 75: 402-409   
  18. Lefebvre JL, Kostadinov D, Chen WV, Maniatis T, Sanes JR (2012) Protocadherins mediate dendritic self-avoidance in the mammalian nervous system. Nature 488: 517-521   
  19. Li Y, Xiao H, Chiou TT, Jin H, Bonhomme B, Miralles CP, Pinal N, Ali R, Chen WV, Maniatis T, De Blas AL (2012) Molecular and functional interaction between protocadherin-gammaC5 and GABAA receptors. J Neurosci 32: 11780-11797   
  20. Kehayova P, Monahan K, Chen WV, Maniatis T (2011) Regulatory elements required for the activation and repression of the protocadherin-alpha gene cluster. Proc Natl Acad Sci U S A 108: 17195-17200   
  21. Cam, H. P. Roles of RNAi in chromatin regulation and epigenetic inheritance. Epigenomics 2(5), 613-626 (2010) Cam, H. P., Noma, K., Ebina, H., Levin, H.L., Grewal, S.I.S. (2008) Host genome surveillance for retrotransposons by transposon-derived proteins. Nature 451, 431-6 
  22. Wang L, Foster M, Zhang Y, Tschantz W, Yang L, Worrall J, Loh C and Xu X. (2008) High yield expression of non-phosphorylated protein tyrosine kinases in insect cells. Protein Expr Purif. 61:204-11 
  23. Cam, H.P., Sugiyama, T., Chen, E.S., Chen, X., FitzGerald, P.C., Grewal, S.I. (2005) Comprehensive analysis of heterochromatin- and RNAi-mediated epigenetic control of the fission yeast genome. Nature Genet. 37, 809-819 
  24. Chen WV, Delrow J, Corrin PD, Frazier JP, Soriano P (2004) Identification and validation of PDGF transcriptional targets by microarray-coupled gene-trap mutagenesis. Nat Genet 36: 304-312   
  25. Chen WV, Soriano P (2003) Gene trap mutagenesis in embryonic stem cells. Methods Enzymol 365: 367-386     


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