Any type of mutations, simple or complex - CRISPR indels, point mutations, conditional knockouts, Cre lines, reporter lines, large fragment deletion, insertion, or replacement - you name it, we make it. Complete service from design to germline transmission.
At least two N1 founders with sequence-validated genotypes will be shipped to customers upon completion of the project, together with a full project report with all genetic data. In addition, the engineered ES cell lines will be stored free of charge for 5 years for future recovery as needed. Additional breeding, genotyping, cryopreservation, cryorecovery and related services are available upon request.
Turnaround time (from design to germline transmission) for most types of mutations is 7-9 months. An additional amount of time may be needed for generating complex mutations, which will be estimated on a case-by-case basis.
A 50% pre-payment will be charged before project initiation, and the remaining balance will be invoiced when N1 founders are ready to ship. Alternative payment plans can be arranged upon request.
Our mouse colonies are serviced by a AAALAC accredited, OLAW-assured vivarium core facility of the Worcester Polytechnic Institute (WPI). Leveragen will coordinate with the WPI vivarium and an approved courier to ship N1 founders to the mouse facility designated by the customer.
Leveragen guarantees the generation and germline transmission of the intended mutation. If N1 animals with the designed mutation are not generated for any technical reason, we will provide a full refund or generate another similar mutation free of charge as per customer's request.